anti ser795 prb (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti ser795 prb
Anti Ser795 Prb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+prb+ser795/Phospho-Rb+(Ser795)+Antibody/pm40614869-71-12-39
Average 94 stars, based on 425 article reviews
Anti Ser795 Prb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+prb+ser795/Phospho-Rb+(Ser795)+Antibody/pm40614869-71-12-39
Average 94 stars, based on 425 article reviews
anti ser795 prb - by Bioz Stars,
2026-11
94/100 stars
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Western Blot:Article Title: The cell-permeable iron chelator M606 inhibits MYCN-driven neuroblastoma via an E2F3-mediated response Article Snippet: M606, DFO, and Exjade (CAS: 201530-41-8 Ontario Chemicals Inc Canada) were dissolved in DMSO. .. Western blotting The primary antibodies were anti-MYCN (1:1000, sc53993, Santa Cruz), anti-HIF-1a (1:1000, ab2185, Abcam), anti-c-MYC (1:500, sc-42, Santa Cruz), anti-RB(IF8) (1:500, sc102, Santa Cruz), anti-p-RB Thr821/826 (1:1000, sc-271930, Santa Cruz), anti-p-RB Ser608 (1:1000, sc-56174, Santa Cruz), anti-p-RB Thr373 (1:1000, sc-16672, Santa Cruz), Incubation:Article Title: The antiproliferative effect of indomethacin-loaded lipid-core nanocapsules in glioma cells is mediated by cell cycle regulation, differentiation, and the inhibition of survival pathways Article Snippet: After electrophoresis, proteins were electrotransferred to nitrocellulose membranes using a semi-dry transfer apparatus (Trans-Blot SD; Bio-Rad Laboratories, Hercules, CA, USA). .. Membranes were incubated for 60 minutes at 4°C in blocking solution (Tris-buffered saline containing 5% nonfat milk and 0.1% Tween 20, pH 7.4) and further incubated with the appropriate primary antibody dissolved in the blocking solution overnight at 4°C.18 The following primary antibodies were used: anti-phospho AKT (Ser473) (pAKT) (1:1000; Cell Signaling, Danvers, MA, USA), anti-AKT (AKT) (1:1000; Cell Signaling), anti-phospho GSK3β (Ser9) (pGSK3β) (1:2000; Cell Signaling), anti-GSK3β (GSK3β) (1:2000; Cell Signaling), anti-GFAP (glial fibrillary acidic protein) (1:2000; Chemicon International), anti-nestin (1:2000; Chemicon International), anti-cyclin D1 (1:1000; Cell Signaling), anti-CDK4 (1:1000; Cell Signaling), anti-CDK6 (1:1000; Cell Signaling), Blocking Assay:Article Title: The antiproliferative effect of indomethacin-loaded lipid-core nanocapsules in glioma cells is mediated by cell cycle regulation, differentiation, and the inhibition of survival pathways Article Snippet: After electrophoresis, proteins were electrotransferred to nitrocellulose membranes using a semi-dry transfer apparatus (Trans-Blot SD; Bio-Rad Laboratories, Hercules, CA, USA). .. Membranes were incubated for 60 minutes at 4°C in blocking solution (Tris-buffered saline containing 5% nonfat milk and 0.1% Tween 20, pH 7.4) and further incubated with the appropriate primary antibody dissolved in the blocking solution overnight at 4°C.18 The following primary antibodies were used: anti-phospho AKT (Ser473) (pAKT) (1:1000; Cell Signaling, Danvers, MA, USA), anti-AKT (AKT) (1:1000; Cell Signaling), anti-phospho GSK3β (Ser9) (pGSK3β) (1:2000; Cell Signaling), anti-GSK3β (GSK3β) (1:2000; Cell Signaling), anti-GFAP (glial fibrillary acidic protein) (1:2000; Chemicon International), anti-nestin (1:2000; Chemicon International), anti-cyclin D1 (1:1000; Cell Signaling), anti-CDK4 (1:1000; Cell Signaling), anti-CDK6 (1:1000; Cell Signaling), Saline:Article Title: The antiproliferative effect of indomethacin-loaded lipid-core nanocapsules in glioma cells is mediated by cell cycle regulation, differentiation, and the inhibition of survival pathways Article Snippet: After electrophoresis, proteins were electrotransferred to nitrocellulose membranes using a semi-dry transfer apparatus (Trans-Blot SD; Bio-Rad Laboratories, Hercules, CA, USA). .. Membranes were incubated for 60 minutes at 4°C in blocking solution (Tris-buffered saline containing 5% nonfat milk and 0.1% Tween 20, pH 7.4) and further incubated with the appropriate primary antibody dissolved in the blocking solution overnight at 4°C.18 The following primary antibodies were used: anti-phospho AKT (Ser473) (pAKT) (1:1000; Cell Signaling, Danvers, MA, USA), anti-AKT (AKT) (1:1000; Cell Signaling), anti-phospho GSK3β (Ser9) (pGSK3β) (1:2000; Cell Signaling), anti-GSK3β (GSK3β) (1:2000; Cell Signaling), anti-GFAP (glial fibrillary acidic protein) (1:2000; Chemicon International), anti-nestin (1:2000; Chemicon International), anti-cyclin D1 (1:1000; Cell Signaling), anti-CDK4 (1:1000; Cell Signaling), anti-CDK6 (1:1000; Cell Signaling), Cell-Signaling:Article Title: The antiproliferative effect of indomethacin-loaded lipid-core nanocapsules in glioma cells is mediated by cell cycle regulation, differentiation, and the inhibition of survival pathways Article Snippet: .. The following primary antibodies were used: anti-phospho AKT (Ser473) (pAKT) (1:1000; Cell Signaling, Danvers, MA, USA), anti-AKT (AKT) (1:1000; Cell Signaling), anti-phospho GSK3β (Ser9) (pGSK3β) (1:2000; Cell Signaling), anti-GSK3β (GSK3β) (1:2000; Cell Signaling), anti-GFAP (glial fibrillary acidic protein) (1:2000; Chemicon International), anti-nestin (1:2000; Chemicon International), anti-cyclin D1 (1:1000; Cell Signaling), anti-CDK4 (1:1000; Cell Signaling), anti-CDK6 (1:1000; Cell Signaling), |